Métodos

Métodos para el muestreo, extracción, procesamiento, e identificación de meiofauna. Más información sobre el uso de meiofauna en investigaciones aplicadas, como evaluaciones de impacto ambiental

Muestreo

Sampling can take two forms either Quantitative or Qualitative. Typically meiofauna are heterogeneously distributed so a larger number of small samples are generally better than few large samples. Before you start sampling be sure to establish a sampling regime appropriate to answering your hypothesis. Also be sure that your replicates are replicates and not pseudoreplicates. Also consider whether you require live or fixed specimens, the identification of some groups, Turbellaria for example, is easier with live specimens. Meiofauna will survive for a reasonable length of time in the sample container, however be sure to remove large macrofauna before transport or they will consume all the oxygen in the sample. Most researchers use formalin (5-10%) for fixing samples.

Quantitative

Muestreo cuantitativo de playas de arena expuestas

Muestreo cuantitativo de planicies marinas

Muestreo cuantitativo de playas rocosas

Sustratos artificiales

Qualitative

Muestreo cualitativo de ambientes sedimentales

Muestreo cualitativo de playas rocosas

Extracción

The methods for extracting meiofauna from the sampled habitat depends on whether the meiofauna are alive or dead. Many meiofaunal groups have mechanisms, such as the duo-gland system, for position maintenance. When the sample contains live fauna the extraction methodology needs a stage which either negates their position maintenance strategy (osmotic shock or anesthetisation) or forces them to move out of the habitat (Uhlig’s method). With fixed samples most extraction methodologies rely on the different settling rates of the meiofauna and the substrate.

Extracción de meiofauna usando decantación simple

Extracción de meiofauna usando la técnica flotación con Ludox

Método de Uhlig con hielo de agua de mar

Anestesia con cloruro de magnesio isotonico

Método de choque osmótica con agua dulce

Procesamiento

Fijación con formalina

Fijación con formalina Fluido de Bouin

Fijación con DESS

Identificación

Morfometricos de Nematodos

Análisis genética

Investigaciones Aplicadas

Evaluaciones de impacto ambientales

Ecotoxicología